A. Sendouda et al., CLONING, NUCLEOTIDE-SEQUENCE AND EXPRESSION OF A BETA-LACTAMASE GENE FROM STREPTOMYCES-LAVENDULAE, FEMS microbiology letters, 112(3), 1993, pp. 343-348
A hybridized DNA fragment was cloned as a 7.6-kb fragment from Strepto
myces lavendulae KCCS0263 using a 1.9-kb SacI- XbaI DNA fragment from
S. cellulosae as a probe. The latter fragment encoded a beta-lactamase
which can bind blue dextran. The hybridized region was reduced to a 2
.8-kb KpnI-BclI fragment and the nucleotide sequence was determined. T
he nucleotide sequence indicated one open reading frame whose amino ac
id sequence is very similar to that of the beta-lactamase from S. cell
ulosae. The gene produced beta-lactamase enzyme at a low but significa
nt amount.