NEOPLASTIC TRANSFORMATION OF A HUMAN PROSTATE EPITHELIAL-CELL LINE BYTHE V-KI-RAS ONCOGENE

Investigations of mechanisms of human prostate carcinogenesis are limi ted by the unavailability of a suitable in vitro model system. We have demonstrated that an immortal, but nontumorigenic, human epithelial c ell line (267B1) established from fetal prostate tissue can be maligna ntly transformed by a biological carcinogen, and can serve as a useful model for investigations of the progression steps of carcinogenesis. Activated Ki-ras was introduced into 267B1 cells by infection with the Kirsten murine sarcoma virus. Morphological alterations and anchorage -independent growth were observed; when cells were injected into nude mice, poorly differentiated adenocarcinomas developed. These findings represent the first evidence of malignant transformation of human pros tate epithelial cells in culture, and support a role for Ki-ras activa tion in a multistep process for prostate neoplastic transformation. (C ) 1993 Wiley-Liss, Inc.