IN-VITRO REGENERATION THROUGH ORGANOGENESIS OF MECONOPSIS-SIMPLICIFOLIA - AN ENDANGERED ORNAMENTAL SPECIES

Authors
SULAIMAN IM BABU CR
Citation
Im. Sulaiman et Cr. Babu, IN-VITRO REGENERATION THROUGH ORGANOGENESIS OF MECONOPSIS-SIMPLICIFOLIA - AN ENDANGERED ORNAMENTAL SPECIES, Plant cell, tissue and organ culture, 34(3), 1993, pp. 295-298
Citations number
8
Categorie Soggetti
Plant Sciences
Journal title
Plant cell, tissue and organ culture → ACNP
ISSN journal
01676857
Volume
34
Issue
3
Year of publication
1993
Pages
295 - 298
Database
ISI
SICI code
0167-6857(1993)34:3<295:IRTOOM>2.0.ZU;2-8
Abstract
Meconopsis simplicifolia (D.Don) Walp. could be propagated by inductio n of adventitious shoots from callus produced on hypocotyl, cotyledon and rosette leaf explants of 4-month-old seedlings. Callus was initiat ed on agar-solidified Murashige and Skoog medium supplemented with 1 m uM kinetin + 10 muM alpha-naphthaleneacetic acid (NAA). Shoots formed when the callus was subcultured on medium supplemented with kinetin or benzyladenine (BA) in combination with NAA, indoleacetic acid, indole butyric acid or gibberellic acid. Excised shoots were rooted on medium containing auxin with 10 muM NAA producing the best rooting (55%).