PRESENCE OF PROTEOLYTIC-ENZYMES IN DICROCOELIUM-DENDRITICUM EGGS

Authors
DEARMASSERRA C GIMENEZPARDO C RODRIGUEZCAABEIRO F
Citation
C. Dearmasserra et al., PRESENCE OF PROTEOLYTIC-ENZYMES IN DICROCOELIUM-DENDRITICUM EGGS, International journal for parasitology, 23(5), 1993, pp. 681-684
Citations number
15
Categorie Soggetti
Parasitiology
Journal title
International journal for parasitology → ACNP
ISSN journal
00207519
Volume
23
Issue
5
Year of publication
1993
Pages
681 - 684
Database
ISI
SICI code
0020-7519(1993)23:5<681:POPIDE>2.0.ZU;2-#
Abstract
The proteolytic activity of eggs from Dicrocoelium dendriticum was exa mined using azocoll as protein substrate. Enzymes from the egg were ca pable of hydrolyzing the protein in a wide range of pH (2, 3 and 4), w ith maximal activity in acid ranges (pH 3-4). These enzymes were sensi tive to serine, cystein, aspartyl and metallo protease inhibitors. Sub sequent analysis by modified SDS-PAGE in gels containing gelatine demo nstrate the presence of three bands with proteolytic activity.