Blastocystis sp. is reported for the first time from faecal samples co
llected from a camel, a llama, a highland bull and a lion in a travell
ing circus. Fresh faecal specimens were examined by light and electron
microscopy, and vacuolar and cyst forms of similar morphology were pr
esent in all three ungulates. These cells were smaller than cultured v
acuolar cells of Blastocystis hominis isolated from humans and contain
ed only a single vacuole in comparison to the multivacuolar cell found
in fresh human faeces. The taxonomic relationship of Blastocystis iso
lated from humans and ungulates remains to be determined. The number o
f parasites present in the hon sample was too small to make valid comp
arisons.