In order to optimize the thermophilic lactic acid fermentation on sacc
harose as the sole carbon source and improve the effectiveness of the
process, alternative nitrogen sources were tested and a minimal broth
composition was found. Of the alternative nitrogen sources, whey prote
in hydrolyzate (WPH) was the best; the broth composition was reduced f
rom seven down to three items. Application of ammonium as a neutralizi
ng agent instead of sodium hydroxide brought an important positive cha
nge.