From 3 404 Ixodes ricinus ticks collected in 12 localities of Styria,
Austria in 1990, 15 tick-borne encephalitis (TBE) virus isolates were
recovered. Minimal field infection rate reached 4.4 virus containing t
icks out of 1 000 collected ticks. Five isolates of TBE virus were obt
ained from target organs of Apodemus flavicollis trapped in locality W
agnitz. In a serosurvey based on virus neutralizing antibodies high pr
evalence of TBE virus was demonstrated in A. flavicollis (47.9 %) and
Clethrionomys glareolus (29.4 %). These rodents formed 57.8 % and 41.0
% of 83 trapped small mammals.