FUNCTIONAL EQUIVALENCE OF COMMON AND UNIQUE SEQUENCES IN THE 3'-UNTRANSLATED REGIONS OF ALFALFA MOSAIC-VIRUS RNA-1, RNA-2, AND RNA-3

The 3' untranslated regions (UTRs) of alfalfa mosaic virus (AMV) RNAs 1, 2, and 3 consist of a common 3'-terminal sequence of 145 nucleotide s (nt) and upstream sequences of 18 to 34 nt that are unique for each RNA. The common sequence can be folded into five stem-loop structures, A to E, despite the occurrence of 22 nt differences between the three RNAs in this region, Exchange of the common sequences or full-length UTRs between the three genomic RNAs did not affect the replication of these RNAs in vivo, indicating that the UTRs are functionally equivale nt, Mutations that disturbed base pairing in the stem of hairpin E red uced or abolished RNA replication, whereas compensating mutations rest ored RNA replication, In vitro, the 3' UTRs of the three RNAs were, re cognized with similar efficiencies by the AMV RNA-dependent RNA polyme rase (RdRp), A deletion analysis of template RNAs indicated that a 3'- terminal sequence of 127 nt in each of the three AMV RNAs was not suff icient for recognition by the RdRp, Previously, it has been shown that this 127-nt sequence is sufficient for coat protein binding, Apparent ly, sequences required for recognition of AMV RNAs by the RdRp are lon ger than sequences required for CP binding.