Em. Mccann et Am. Lever, LOCATION OF CIS-ACTING SIGNALS IMPORTANT FOR RNA ENCAPSIDATION IN THELEADER SEQUENCE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2, Journal of virology, 71(5), 1997, pp. 4133-4137
We used a series of deletion mutations in the untranslated leader regi
on of human immunodeficiency virus type 2 (HIV-2) to seek cis-acting p
ackaging signals, Sequences between the 5' major splice donor and the
gag initiation codon, where such signals have been identified in HIV-1
, appear to make a measurable but very minor contribution to genomic R
NA packaging, and deletions here had little effect on viral replicatio
n in vitro, Immediately 5' to the splice donor, two regions were ident
ified which, when deleted, caused a significant replication defect, Th
e most proximal of these to the splice donor demonstrated a phenotype
consistent with its being a major cis-acting packaging signal in HIV-2
.