LYMPHOKINE GENE-TRANSCRIPTION IN CD4- T-CELLS OF A TYPE-III BARE LYMPHOCYTE SYNDROME PATIENT(CD8)

In this study we analyzed the impact of a MHC class-II-deficient envir onment on the differentiation of CD4+ CD8- T lymphocytes into function al defined subsets of lymphokine-producing T-helper cells. To this end a CD4+ CD8- T-cell line and CD4+ CD8- T-cell clones, isolated from PB MCs of a type III BLS patient, were stimulated in vitro with anti-CD3 and PMA and assessed for lymphokine transcription patterns. The result s of these analyses show that CD4+ CD8- T cells that have matured in a MHC class-II-deficient environment display lymphokine transcription p atterns that resemble those of MHC class-II-expressing family control- derived CD4+ CD8- T cells.