CHARACTERIZATION OF ESCHERICHIA-HERMANNII BY RIBOSOMAL DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM

Authors
PICARDPASQUIER N PICARD B KRISHNAMOORTHY R GOULLET P
Citation
N. Picardpasquier et al., CHARACTERIZATION OF ESCHERICHIA-HERMANNII BY RIBOSOMAL DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM, Research in microbiology, 144(6), 1993, pp. 485-488
Citations number
6
Categorie Soggetti
Microbiology
Journal title
Research in microbiology → ACNP
ISSN journal
09232508
Volume
144
Issue
6
Year of publication
1993
Pages
485 - 488
Database
ISI
SICI code
0923-2508(1993)144:6<485:COEBRD>2.0.ZU;2-9
Abstract
Ribosomal DNA polymorphism was used to characterize strains of Escheri chia hermannii and to differentiate them from E. coli. DNA from 11 E. hermannii strains previously separated into three zymotypes by enzyme electrophoretic polymorphism was digested with HindIII and EcoRI restr iction enzymes and analysed by Southern blotting. The 10 ribotypes obt ained with EcoRI fell into 3 groups which correlated with the correspo nding zymotypes, and the 5 ribotypes obtained with HindIII were clearl y distinct from those of E. coli strains.