Gc. Packard et Mj. Packard, DELAYED INOCULATIVE FREEZING IS FATAL TO HATCHLING PAINTED TURTLES (CHRYSEMYS-PICTA), Cryo-letters, 14(5), 1993, pp. 273-284
Hatchling painted turtles from northcentral Nebraska were placed indiv
idually into artificial nests in jars of damp soil and then were expos
ed to subzero temperatures using an ecologically realistic protocol. S
ome animals froze during the tests, but none of these turtles survived
. Instead, animals that tolerated subzero temperatures remained unfroz
en and sustained a state of supercooling. Skin of the turtles resisted
the propagation of ice into body compartments from the environment, b
ut many animals nonetheless were inoculated after a delay that was inf
luenced both by the duration of exposure and by.the minimum temperatur
e experienced during the test.