The osmotic volume response of human spermatozoa was determined using
a method that is independent of cell shape, namely, an electron parama
gnetic resonance (EPR) technique. The results yield a Boyle van't Hoff
plot which is linear in the range 250 to 1500 milliosmolal (mOsm) of
sodium chloride (r2 = 0.996). Thirteen percent of the isotonic water w
as found to be osmotically inactive.