C. Lohning et al., ISOLATION OF THE TYE2 GENE REVEALS ITS IDENTITY TO SW13 ENCODING A GENERAL TRANSCRIPTION FACTOR IN SACCHAROMYCES-CEREVISIAE, Current genetics, 24(3), 1993, pp. 193-199
The TYE2 gene was identified by recessive mutations which result in a
significant reduction of Ty-mediated ADH2 expression. We cloned the TY
E2 gene and analyzed its sequence. A large open reading frame of 825 c
odons was found encoding a rather hydrophilic, 93-kilodalton protein w
hich contains a highly acidic region at its N-terminus. By sequence co
mparison we found that TYE2 is identical to gene SWI3 which has recent
ly been shown to encode a nuclear protein which may function as a glob
al transcription activation factor. The TYE2/SWI3 protein is necessary
for the initiation of Ty1 transcription at its major initiation site
in the delta element. Furthermore TYE2 function seems to be important
for the expression of a variety of Ty-unrelated functions such as ADH1
expression, sporulation, growth on maltose, galactose, raffinose, and
on non-fermentable carbon sources.