TRANSFORMATION OF TRICHODERMA-REESEI WITH THE HORMOCONIS-RESINAE GLUCOAMYLASE-P (GAMP) GENE - PRODUCTION OF A HETEROLOGOUS GLUCOAMYLASE BY TRICHODERMA-REESEI

A cDNA encoding for the glucoamylase P enzyme (GAMP) of the fungus Hor moconis resinae was introduced into the cellulolytic filamentous fungu s Trichoderma reesei under the control of the promoter of the major ce llulase gene (cbh1) of Trichoderma. The transforming vector plasmid us ed was found to be integrated into the genome of T reesei at various l ocations and in multiple copies. The size of the GAMP secreted by Tric hoderma varied because of different glycosylation patterns. The best t ransformant strains secreted about 700 mg/l of active GAMP, which is 2 0-fold more than obtained with H. resinae.