THE PREPARATION OF FIBRINOGEN CONCENTRATE FOR USE AS FIBRIN GLUE BY 4DIFFERENT METHODS

Fibrinogen concentrates for use as fibrin glue were prepared by modifi cation of a cryoprecipitate method. The goals were the optimization of a method for different centrifuges and anticoagulants and the assay o f factors not previously analyzed. Following a -70-degrees-C freeze an d a 4-degrees-C thaw, CPDA-1 and ACD plasma were centrifuged at 6500 x g for 5 minutes or, alternatively, at 5000 x g for 7 minutes. The sup ernatant plasma was expressed to a final volume of 15.5 +/- 3 mL, and concentrates were stored at -30-degrees-C. Preconcentration and postco ncentration samples were analyzed for fibrinogen, fibronectin, factor XIII, and plasminogen content. Fibrinogen in CPDA-1 plasma was signifi cantly higher than that in ACD plasma both before and after concentrat ion at both centrifugation speeds. Fibronectin, factor XIII, and plasm inogen concentrations were not significantly affected by centrifugatio n speed or the type of anticoagulant used. Fibronectin and plasminogen concentrations were significantly increased in components that were h eld for 5 to 6 days, as compared to those held for 0 to 1 day before f reezing. Storage for up to 20 days in CPDA-1 and up to 5 months in ACD did not affect analyte concentration. It is concluded that ACD plasma centrifuged at 5000 x g yields a significantly low concentration of f ibrinogen, while CPDA-1 plasma centrifuged at 6500 x g yields the high est amount. Acceptable yields were obtained from centrifugation of ACD plasma at 6500 x g and of CPDA-1 plasma at 5000 x g for use as fibrin glue.