NEW ENZYME BIOSENSOR FOR DETERMINATION OF GLUCOSE

A simple technique has been applied to the development of a glucose se nsor by entrapping the glucose oxidase into a polymer matrix deposited on the surface of a platinum electrode by electropolymerization of N- methylpyrrole in aqueous solutions containing enzyme, altion and tiron . The new sensor is characterized with fast response time (about 30 s) at high enough stability on storage and operation (> 45 days and > 30 measurements, respectively). In the presence of dioxygen, the electro de current response changes linearly with glucose concentration within the range 0.1-6 mM and does not reach any limit even at 15 mM. The ro le of altion and tiron in electropolymerization and glucose determinat ion is discussed.