BIOSYNTHESIS OF VITAMIN-B12 - DISCOVERY OF THE ENZYMES FOR OXIDATIVE RING CONTRACTION AND INSERTION OF THE 4TH METHYL-GROUP

Authors
SCOTT AI ROESSNER CA STOLOWICH NJ SPENCER JB MIN C OZAKI SI
Citation
Ai. Scott et al., BIOSYNTHESIS OF VITAMIN-B12 - DISCOVERY OF THE ENZYMES FOR OXIDATIVE RING CONTRACTION AND INSERTION OF THE 4TH METHYL-GROUP, FEBS letters, 331(1-2), 1993, pp. 105-108
Citations number
19
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
331
Issue
1-2
Year of publication
1993
Pages
105 - 108
Database
ISI
SICI code
0014-5793(1993)331:1-2<105:BOV-DO>2.0.ZU;2-I
Abstract
In the vitamin B-12 biosynthetic pathway the enzymes responsible for t he conversion of precorrin-3 to precorrin-4 have been identified as th e gene products of cobG and cobJ from Pseudomonas denitrificans. CobG catalyzes the oxidation of precorrin-3 to precorrin-3x (a hydroxy lact one) whereas CobJ is a SAM-dependent C-17 methyl transferase and is ne cessary for ring contraction. A mechanism for ring contraction is prop osed.