PROTEIN YB-1 INTERACTING WITH HUMAN PAPILLOMA-VIRUS TYPE-18 ENHANCER - IN-VIVO IDENTIFICATION WITH ANTIBODIES TO A SYNTHETIC PEPTIDE

Clones containing DNA homologous to the YB-1 gene were obtained from a HeLa cell cDNA library using lambdagt11 phage as the vector and the b inding of human papilloma virus (HPV) type 18 DNA enhancer to nitrocel lulose-bound protein probes as the test reaction. The YB-1 gene encode s a protein which can bind to the regulatory region of the Y genes of the type II major histocompatibility complex (HLA II). The YB-1 transc ripts were found in all cervical carcinomas tested. Based on a compute r analysis, a peptide was synthesized and used after conjugation with hemocyanin to raise antibodies in rabbit. The serum obtained helped to identify a nuclear 42-kDa protein in normal human fibroblasts and HeL a cells.