FAST DETERMINATION OF WHOLE-BLOOD GLUCOSE WITH A CALORIMETRIC MICRO-BIOSENSOR

Fast determination of whole blood glucose without any pretreatment was achieved using a calorimetric FIA micro-biosensor. Glucose oxidase wa s covalently immobilized, together with catalase, onto controlled pore glass beads and packed into a micro-column. By reducing the sample vo lume down to 1 mul, a measurement period of about 40 s and an enlarged linear range of 0.5-20 mM glucose were obtained. The cor-relation coe fficient was 0.98 when the micro-biosensor was compared to the Reflolu x-S blood glucose analyser. The relative standard deviation for 100 bl ood samples was 3.7%.