CHARACTERIZATION OF THE ANTIGENIC DETERMINANTS OF THE LEISHMANIA-INFANTUM HISTONE H3 RECOGNIZED BY ANTIBODIES ELICITED DURING CANINE VISCERAL LEISHMANIASIS
M. Soto et al., CHARACTERIZATION OF THE ANTIGENIC DETERMINANTS OF THE LEISHMANIA-INFANTUM HISTONE H3 RECOGNIZED BY ANTIBODIES ELICITED DURING CANINE VISCERAL LEISHMANIASIS, Clinical and experimental immunology, 106(3), 1996, pp. 454-461
In the present study we show that sera from dogs naturally infected wi
th the protozoan parasite Leishmania infantum contain antibodies that
specifically react with the parasite histone H3. Using synthetic pepti
des covering the complete sequence of the protein we located the linea
r antigenic determinants within the 40 amino-terminal amino acids of t
he molecule. In addition to the complete form of the protein (rLiH3),
two regions of the Leishmania histone H3 were expressed as recombinant
proteins: the rLiH3-Nt fragment containing the 39 amino-terminal amin
o acids and the rLiH3-Ct fragment containing the 90 carboxyl-terminal
residues. Competition experiments using the protein fragment rLiH3-Nt
as competitor confirmed that the antigenic determinants of histone H3
are confined to the amino-terminal domain. This domain, which is belie
ved to be exposed on the nucleosome surface, is also the most evolutio
narily divergent region of the L. infantum histone H3. Visceral leishm
aniasis (VL) sera do not react with mammalian histones, an indication
that the anti-histone response elicited during Leishmania infection is
triggered by the parasite histone. The results of the prevalence of a
nti-histone H3 antibodies in canine VL sera together with the sequence
-specific characteristics of the amino-terminal region of L. infantum
histone H3 indicate that the recombinant protein rLiH3-Nt may be of us
e for diagnosis of canine VL.