RECEPTOR PROTEIN-TYROSINE KINASE DDR IS UP-REGULATED BY P53 PROTEIN

We have previously reported on radiation-induction of ptk-3 in rat ast rocyte culture [Sakuma et al. (1995) Radiat, Res. 143, 1-7]. Ptk-3 was considered to be a rat version of human DDR (discoidin domain recepto r). We cloned and analyzed genomic DNA of the DDR and its promoter reg ion, We discovered that the promoter region contained a consensus sequ ence of the p53 tumor suppressor binding site. Adenovirus-mediated p53 transfection induced a high level of DDR mRNA in SAOS2 human osteosar coma cells. These results indicate that DDR is up-regulated by the p53 protein.