BANDEIRAEA-(GRIFFONIA)-SIMPLICIFOLIA LECTIN-I, ISOLECTIN-A(4), REACTING WITH TN (GA1NAC-ALPHA-1-]SER THR) OR GALABIOSE (GA1-ALPHA-L-]4GA1) CONTAINING LIGANDS/

Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A(4)(GS I-A(4 )) reacting with the Tn(GalNAc alpha 1 --> Ser/Thr) sequence or human blood group P-k active disaccharide (E, Gal alpha 1 --> 4Gal, galabios e) was studied by quantitative precipitin (QPA) and precipitin-inhibit ion assays. When human blood group P-1 or Tn active glycoproteins were tested by QPA, GS I-A(4) reacted strongly with both the Tn active gly coproteins purified from asialo porcine, ovine and armadillo submandib ular glands and a P-1 active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added, The as ialo porcine salivary glycoprotein-GS I-A(4) interaction was inhibited by both Tn containing glycopeptides and Gal alpha 1 --> 4Gal indicati ng that GS I-A(4) not only reacts with human blood group A(GalNAc alph a 1 --> 3Gal) and B(Gal alpha 1 --> 3Gal) active disaccharides, but al so recognizes the Tn sequence and the E(Gal alpha 1 --> 4Gal) ligand, From these results, the carbohydrate specificity of GS I-A(4) can be d efined as A, Tn greater than or equal to B and E.