SPECIFIC BINDING OF NUCLEAR PROTEINS TO REGULATORY REGION OF RAT TRYPTOPHAN OXYGENASE GENE

The specific binding of rat liver nuclear proteins to fragments contai ning nucleotides 466-292 and 292-178 relative to the transcription sta rt site of the rat tryptophan oxygenase gene was analyzed by electroph oretic mobility. Studies of the competition with a synthetic consensus sequence for the nuclear factor 1 (NF 1) recognition site showed that the liver nuclear proteins responsible for the formation of specific complexes with these fragments belong to the NF 1 family. Transacting factors of the NF 1 family also form several complexes with each of th e two tested fragments of the tryptophan oxygenase gene.