12-0-TETRADECANOYLPHORBOL 13-ACETATE (TPA)-INDUCED ENZYMATIC CHANGE OF HUMAN NON-T, NON-B LYMPHOID LEUKEMIA-CELL LINES

Culture of two lymphoid leukemia cell lines with 5 x 10(-9) to 10(-7) M 12-0-tetradecanoyl-phorbol 13-acetate (TPA) induced the significant increase in sialyltransferase, acid phosphatase and butyrate esterase activities, the decrease in poly(A) polymerase and terminal deoxynucle otidyl transferase (TdT) activities. B4-, J5-, TdT- or PNA-positive ce lls were decreased in TPA-treated cells, while B1-positive cells were increased. These results suggest enzymatic changes as an aspect of TPA -induced differentiation in lymphoid leukemia cell lines. These enzyma tic activities may be useful markers for the differentiation of lympho id leukemia cells.