ENHANCED SECRETION OF TUMOR-NECROSIS-FACTOR-ALPHA, IL-6, AND IL-1-BETA BY ISOLATED LAMINA PROPRIA MONONUCLEAR-CELLS FROM PATIENTS WITH ULCERATIVE-COLITIS AND CROHNS-DISEASE
Hc. Reinecker et al., ENHANCED SECRETION OF TUMOR-NECROSIS-FACTOR-ALPHA, IL-6, AND IL-1-BETA BY ISOLATED LAMINA PROPRIA MONONUCLEAR-CELLS FROM PATIENTS WITH ULCERATIVE-COLITIS AND CROHNS-DISEASE, Clinical and experimental immunology, 94(1), 1993, pp. 174-181
The perpetuation of inflammation in ulcerative colitis and Crohn's dis
ease may be regulated in part by an increased secretion of proinflamma
tory cytokines due to either an appropriate response to initial stimul
ating agents, and/or due to an impaired down-regulation of cytokine se
cretion. The aim of this study was to determine the secretion patterns
of the proinflammatory cytokines tumour necrosis factor-alpha (TNF-al
pha), IL-6 and IL-1beta, from isolated lamina propria mononuclear cell
s (LPMNC) isolated from colonic biopsies from patients with untreated
ulcerative colitis or Crohn's disease. LPMNC isolated from involved in
flammatory bowel disease (IBD) mucosa spontaneously produced increased
amounts of TNF-alpha, IL-6, and IL-1beta. The TNF-alpha secretion fro
m IBD LPMNC could be further enhanced by pokeweed mitogen stimulation.
The secretion patterns of TNF-alpha and IL-1beta by LPMNC from patien
ts with either ulcerative colitis or Crohn's disease demonstrated a cl
ose correlation with the degree of tissue involvement and mucosal infl
ammation. LPMNC from non-involved ulcerative colitis mucosa secreted m
arkedly increased levels of IL-6 compared with non-involved Crohn's di
sease mucosa or control mucosa. The heightened IL-6 secretion from LPM
NC from non-involved ulcerative colitis mucosa without visible or micr
oscopic signs of inflammation indicates that the pathophysiologic mech
anisms involved in the initiation of inflammation may differ between u
lcerative colitis and Crohn's disease. The determination of proinflamm
atory cytokine secretion by isolated LPMNC from colonoscopic biopsies
may be a sensitive method for monitoring the severity of mucosal infla
mmation in IBD patients.