COMPLETE DNA-SEQUENCE OF CANINE ADENOVIRUS TYPE-1

The complete DNA sequence of a field strain of canine adenovirus type 1 was determined by sequencing random fragments of viral DNA cloned in to pBluescript. The virus has a genome of 30 536 bp Ranked by two iden tical 161 bp inverted terminal repeats. Thirty ORFs have been identifi ed, based on genomic location or sequence identity with published aden oviruses, These are arranged into similar discrete regions found in th e human adenoviruses. ORFs in the late region show greatest identity w ith published human adenovirus sequences, whereas the E3 and E4 ORFs s how little or none.