AN INHIBITORY EFFECT OF ACTIN ON CASEIN KINASE-II ACTIVITY IN-VITRO

The inhibitory effect of actin on protein phosphorylation by three dis tinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of alpha- monomeric CK-II (CK-II alpha) as well as oligomeric CK-II (alpha(2) be ta(2)) in a dose-dependent manner, but has no effect on the activities of the two other kinases; and (ii) actin-induced inhibition of CK-II activity is due to the binding of actin to the alpha-subunit of CK-II and is non-competitive with its phosphate accepters. In addition, it i s demonstrated that actin binds directly to CK-II: both actin and CK-I I are coprecipitated by anti-serum against Drosophila CK-II beta or by specific IgG against Ascaris suum muscle actin. The results presented here suggest that actin can suppress CK-II-mediated signal transducti on.