CYROMAZINE RESISTANCE IN DROSOPHILA-MELANOGASTER (DIPTERA, DROSOPHILIDAE) GENERATED BY ETHYL METHANESULFONATE MUTAGENESIS

Flies resistant to cyromazine (CGA-72662) were selected in susceptible laboratory populations of Drosophila melanogaster (Meigen) treated wi th ethyl methane-sulfonate after growth on cyromazine concentrations > LC99. Two resistant lines were obtained. In each case, resistance was a result of a mutation in a single, but different, gene. The resistanc e genes, designated Rst(2)Cyr and Rst(3)Cyr, were localized to map pos itions 64 on chromosome II and 47 on chromosome III, respectively. Con centration-mortality analysis of each mutant revealed that both genes conferred a low level (<5 times) of resistance to cyromazine. Rst(2)Cy r produced LC99s of 1.3 x 10(-4)% (wt/vol) for heterozygotes and 2.7 x 10(-4)% for homozygotes; Rst(3)Cyr values were 1.6 x 19(-4) and 1.8 x 10(-4)%, respectively. These values compare with an LC99 of 5 x 10(-5 )% for wild-type. The role of D. melanogaster as a model for insectici de resistance studies is discussed, especially the comparison of labor atory-generated cyromazine resistance in D. melanogaster with field re sistance in Musca domestica L.