EFFECT OF VINASSE ON GERMINATION OF METARHIZIUM-ANISOPLIAE (METSCH) SOROKIN IN-VITRO AND IN THE SOIL

The effect of vinasse on Metarhizium anisopliae spores germination ''i n vitro'' and their survival after addition of the residue in the soil was studied. The germination was evaluated at 8, 16 and 24 hours inte rvals in different treatments (Vinasse pH 3.5, 6.5 and Potato/Dextrose medium control). M. anisopliae survival in soil with vinasse was obse rved 15 and 45 days after the soil inoculation. The results showed tha t vinasse inhibited the germination of M. anisopliae in 55.76 and 42.6 9%, respectively, in the pH 3.5 and 6.5. The application of vinasse to the soil artificially inoculated with M. anisopliae reduced significa tively the number of colonies recovered and altered qualitatively the environmental microbiota in the studied area.