CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE CDNA-ENCODING HUMANCARDIAC TROPONIN-I

Authors
ARMOUR KL HARRIS WJ TEMPEST PR
Citation
Kl. Armour et al., CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE CDNA-ENCODING HUMANCARDIAC TROPONIN-I, Gene, 131(2), 1993, pp. 287-292
Citations number
22
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
131
Issue
2
Year of publication
1993
Pages
287 - 292
Database
ISI
SICI code
0378-1119(1993)131:2<287:CAEIEO>2.0.ZU;2-F
Abstract
We have cloned and sequenced the human cardiac troponin I (cTnI)-encod ing cDNA with the aim of expressing the cDNA in Escherichia coli. The cDNA was successfully expressed as a fusion product with beta-galactos idase and as an unfused protein. Both polypeptides were recognised by an anti-human cTnI antibody.