Dv. Marques, INDUCTION OF SOMATIC EMBRYOGENESIS ON COFFEA-EUGENIOIDES MOORE BY IN-VITRO CULTURE OF LEAF EXPLANTS, Cafe, cacao, the, 37(3), 1993, pp. 251-255
The induction of Low Frequency Somatic Embryogenesis (LFSE) and High F
requency Somatic Embryogenesis (HFSE) has been performed in Coffea eug
enioides Moore by in vitro culture of leaf explants. From the five cul
ture media (B5, MS, N30K, SH and W) assayed with similar levels of gro
wth regulators only White medium conducted to somatic embryogenesis. T
herefore, along with the already known influence of 2,4-D, the mineral
composition of the culture medium appeared to condition the capacity
of somatic embryogenesis in coffee.