Nucleases are important analytical enzymes and are used widely for the
determination of nucleic acid structure. Their application depends on
the specificity and mode of action of the particular enzyme. Nuclease
s have also found application in the production of flavor enhancers li
ke 5'IMP and 5'GMP, removal of nucleic acids in single cell protein pr
eparations, and as therapeutic agents. Immobilization of nucleases and
the use of immobilized nucleases for various biotechnological applica
tions have been reviewed.