METABOLISM OF ANILINE AND 3-CHLOROANILINE IN PSEUDOMONAS-ACIDOVORANS CA28 - EVIDENCE OF ISOFUNCTIONAL MUCONATE CYCLOISOMERASES

Two distinct muconate cycloisomerases are involved in the degradation of aniline and 3-chloroaniline (3-CA) proceeding via the ortho-cleavag e pathways in Pseudomonas acidovorans CA28. After partial purification of these two enzymes kinetic investigations resulted in a clear diffe rentiation of aniline-derived muconate cycloisomerase (MC) and 3-CA-de rived chloromuconate cycloisomerase (CMC). A further result of this st udy revealed the simultaneous coexistence of MC and CMC in strain CA28 when grown on a mixture of aniline and 3-CA.