A RAPID METHOD OF EVALUATING PERMEABILIZING ACTIVITY AGAINST PSEUDOMONAS-AERUGINOSA

Authors
AYRES H FURR JR RUSSELL AD
Citation
H. Ayres et al., A RAPID METHOD OF EVALUATING PERMEABILIZING ACTIVITY AGAINST PSEUDOMONAS-AERUGINOSA, Letters in applied microbiology, 17(4), 1993, pp. 149-151
Citations number
8
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
17
Issue
4
Year of publication
1993
Pages
149 - 151
Database
ISI
SICI code
0266-8254(1993)17:4<149:ARMOEP>2.0.ZU;2-4
Abstract
A rapid (< 30 min) spectrophotometric method has been used to assess t he ability of chemical agents to permeabilize the outer membrane of Ps eudomonas aeruginosa. When permeabilized, the cells are rendered readi ly sensitive to lysozyme, an enzyme that is normally excluded from the peptidoglycan of this organism. In addition to ethylenediamine tetraa cetic acid, substances showing permeabilizing activity were citric, is ocitric, malic and gluconic acids (but not gluconic acid lactone) and sodium polyphosphate.