H. Ayres et al., A RAPID METHOD OF EVALUATING PERMEABILIZING ACTIVITY AGAINST PSEUDOMONAS-AERUGINOSA, Letters in applied microbiology, 17(4), 1993, pp. 149-151
A rapid (< 30 min) spectrophotometric method has been used to assess t
he ability of chemical agents to permeabilize the outer membrane of Ps
eudomonas aeruginosa. When permeabilized, the cells are rendered readi
ly sensitive to lysozyme, an enzyme that is normally excluded from the
peptidoglycan of this organism. In addition to ethylenediamine tetraa
cetic acid, substances showing permeabilizing activity were citric, is
ocitric, malic and gluconic acids (but not gluconic acid lactone) and
sodium polyphosphate.