IMMUNIZATION WITH A PLASMID EXPRESSING PNEUMOCOCCAL SURFACE PROTEIN-A(PSPA) CAN ELICIT PROTECTION AGAINST FATAL INFECTION WITH STREPTOCOCCUS-PNEUMONIAE

Pneumococcal surface protein A (PspA) is a protection-eliciting protei n of Streptococcus pneumoniae. We observed that immunization of BALB/c mice with a plasmid expressing PspA significantly protected the mice from lethal challenge with S. pneumoniae when compared to control mice that received injections of the plasmid vector alone. The plasmid con struct expressing PspA has been designated pKSD2601. Mice immunized in tramuscularly with pKSD2601 had a mean log of colony-forming units of 2.97+/-0.25 pneumococci circulating in their blood at 24 h after chall enge as compared with control mice that had a mean log of colony-formi ng units of 4.95+/-0.59. Those mice with lower numbers of pneumococci subsequently survived the challenge. Given the quantitative nature and ultimate end point (ie live versus dead) our mouse model should be us eful in working out optimum expression of bacterial genes for DNA immu nization.