A new casting technique for studying the three-dimensional structure o
f the human retinal vascular system on post mortem tissue was develope
d. The technique was designed to provide an overview presentation of t
he retinal vasculature such that localized areas with vascular patholo
gy could be identified for further analysis of three-dimensional struc
ture. The paper presents qualitative characteristics of three-dimensio
nal structure in the normal human retinal vascular bed, to provide a b
asis for future studies on vascular impairment in retinal disease.