An introduction is given to the literature concerning methods and obje
ctives for cytometric DNA analysis of plant nuclei. This area has gain
ed relevance with applications in plant breeding and seed production i
ndustries, where laboratories unfamiliar with cytometry are adopting t
he method. An extensive graphical guide to interpreting DNA histograms
and their problems is given. Conversely, cytometry laboratories unfam
iliar with plant sciences will find herein a guide, and references, to
adapt their methods to plant material. A table of 2C values reassesse
d by flow cytometry for 70 plant species, plus the genome composition
(GC%) in many instances, is also included.