EXPERIMENTAL CONSEQUENCES OF DIFFERENCES BETWEEN COMPOSITION OF THE SAMPLE SOLVENT AND OF THE MOBILE-PHASE IN HPLC

Anomalous peaks may be expected to occur even in cases when sample sol ubility in mobile phase is sufficient if sample solvent and mobile pha se differ considerably in viscosity and/or elution power of sample sol vent exceeds mobile phase strength substantially. Then, to improve pea k shapes, the sample solvent - mobile phase viscosity ratio should be kept fairly below two and too high elution power of the sample solvent should be decreased by mixing with mobile phase prior to injection.