EFFECT OF CARBON CONTENT BONDED ON SILICA SURFACE ON RETENTION VALUESIN REVERSED-PHASE LIQUID-CHROMATOGRAPHY AND ION-PAIR LIQUID-CHROMATOGRAPHY

Effect of the carbon content on the stationary surface (N(S)) on reten tion values in reversed-phase liquid chromatography (RPLC) and ion-pai r liquid chromatography (RP-IPC) has been investigated. The k(RP)' and k(RP)' values are linearly related with N in RPLC and RP-IPC. The lin ear relationship almost passes through the origin in the former case, but does not pass through origin in the latter case. There is a strong er effect of carbon content on the retention in RP-IPC than that in RP LC. It can be concluded that the retention mechanism of RP-IPC is diff erent from that of RPLC. Effect of the carbon content on the stationar y surface on retention in RP-IPC can be explained by the electrostatic model, which also confirms the validity of the electrostatioc model f or RP-IPC.