ON THE STRUCTURE OF ELECTRICAL DOUBLE-LAYER OF IGG IMMOBILIZED ON POLYSTYRENE MICROSPHERES

Citation
F. Galisteogonzalez et al., ON THE STRUCTURE OF ELECTRICAL DOUBLE-LAYER OF IGG IMMOBILIZED ON POLYSTYRENE MICROSPHERES, Journal of biomaterials science. Polymer ed., 4(6), 1993, pp. 631-641
Citations number
22
Categorie Soggetti
Material Science","Engineering, Biomedical
ISSN journal
09205063
Volume
4
Issue
6
Year of publication
1993
Pages
631 - 641
Database
ISI
SICI code
0920-5063(1993)4:6<631:OTSOED>2.0.ZU;2-X
Abstract
Latex particles used in the development of immunoassays usually presen t stability problems when the antibody is attached to the surface. Thi s work is an attempt to know the potential distribution parameters aro und these complexes by electrokinetic measurements. The conversion of mobility data into zeta-potential was carried out by different theoret ical approaches developed by Smoluchowski (classical equation), O'Brie n and White, and Dukhin and Semenikhin. The zeta-potential calculated with allowance for electrical double layer (e.d.l.) polarization was s ubstantially greater than zeta calculated according to the classical e lectrokinetic theory. The greater values of zeta(D-S) in comparison wi th zeta(O-W) were readily explained on the basis that in the first the ory, the contribution to polarization from all ions of the diffuse lay er was taken into account, whereas O'Brien and White accounted for onl y the ions of the hydrodynamically mobile part of the e.d.l. This is a n indication that the anomalous surface conductance of latex particles coated by IgG molecules is much higher than that of bare latex partic les.