PRODUCTION AND PROPERTIES OF A FACTOR-X CELLULOSE-BINDING DOMAIN FUSION PROTEIN

Authors
ASSOULINE Z SHEN H KILBURN DG WARREN RAJ
Citation
Z. Assouline et al., PRODUCTION AND PROPERTIES OF A FACTOR-X CELLULOSE-BINDING DOMAIN FUSION PROTEIN, Protein engineering, 6(7), 1993, pp. 787-792
Citations number
27
Categorie Soggetti
Biology
Journal title
Protein engineering → ACNP
ISSN journal
02692139
Volume
6
Issue
7
Year of publication
1993
Pages
787 - 792
Database
ISI
SICI code
0269-2139(1993)6:7<787:PAPOAF>2.0.ZU;2-#
Abstract
A fusion protein, FX-CBD(Cex), which comprises factor X with a cellulo se-binding domain (CBD(Cex)) fused to its C-terminus, was produced in BHK cells. It was purified from the culture medium by affinity chromat ography on cellulose. FX-CBD(Cex) could be activated to FXa-CBD(Cex) w ith Russell viper venom. FXa-CBD(Cex) was as active as FXa against a c hromogenic substrate and against proteins containing the Ile - Glu - G ly - Arg sequence hydrolysed by FXa. FXa-CBD(Cex) retained its activit y when adsorbed to cellulose.