HPLC SEPARATIONS OF NORMAL AND MODIFIED NUCLEOBASES AND NUCLEOSIDES ON AN AMINO SILICA-GEL COLUMN

Authors
RAVANAT JL DOUKI T INCARDONA MF CADET J
Citation
Jl. Ravanat et al., HPLC SEPARATIONS OF NORMAL AND MODIFIED NUCLEOBASES AND NUCLEOSIDES ON AN AMINO SILICA-GEL COLUMN, Journal of liquid chromatography, 16(15), 1993, pp. 3185-3202
Citations number
20
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of liquid chromatography → ACNP
ISSN journal
01483919
Volume
16
Issue
15
Year of publication
1993
Pages
3185 - 3202
Database
ISI
SICI code
0148-3919(1993)16:15<3185:HSONAM>2.0.ZU;2-T
Abstract
An amino substituted silica gel column was used for the normal phase H PLC separation of nucleic acid components, including free bases and th e corresponding 2'-deoxyribo- and ribonucleosides. The chromatographic system was also found to be particularly convenient for the separatio n of various modified nucleosides, including oxidation products of 2'- deoxyguanosine and pyrimidine components, thymidine cyclobutane dimers , dinucleoside monophosphate photoproducts and pyrimidine (6-4) pyrimi done adducts.