ELECTROPOLYMERIZED 1,3-DIAMINOBENZENE FOR THE CONSTRUCTION OF A 1,1'-DIMETHYLFERROCENE MEDIATED GLUCOSE BIOSENSOR

An electron-mediated glucose biosensor was constructed from spectrosco pic-grade graphite electrodes in three steps. A non-conducting electro polymerized film serves to screen out electroactive interferents and p revents electrode fouling. The electron mediator 1,1'-dimethylferrocen e is adsorbed on, and retained by the polymer film-modified electrode. Glucose oxidase is then crosslinked with glutaraldehyde on the modifi ed electrode. This yields a biosensor with linear glucose response (2- 76 mM) at + 150 mV vs. SCE, and little or no interference from oxygen, with a lifetime of up to four months.