INTEGRATED ENZYME REACTOR DETECTOR FOR THE DETERMINATION OF MULTIPLE SUBSTRATES BY IMAGE-ANALYSIS

An image analyzer system was used to develop a multicomponent analysis method for enzyme substrates. Image analysis provides a technique whi ch can spatially resolve closely spaced responses, thus allowing for t he detection of multiple substrates with a single reactor/detector. Fo r successful implementation of this method a procedure for immobilizin g large amounts of enzyme activity onto a highly crosslinked polydextr an support (> 300 U/ml gel) was devised. A microsized integrated react or/detector (< 4.5 mul) was constructed by packing the insoluble enzym es into a small capillary. Using this new technique, the concentration s of mixtures containing glucose and glucose-6-phosphate (0-8 mM) were predicted using immobilized glucose oxidase and glucose-6-phosphate d ehydrogenase.