SURFACE-PLASMON RESONANCE SENSOR AS A DETECTOR IN HPLC AND SPECIFIC LACTATE DETERMINATION

Authors
CASTILLO JR CEPRIA G DEMARCOS S GALBAN J MATEO J RUIZ EG
Citation
Jr. Castillo et al., SURFACE-PLASMON RESONANCE SENSOR AS A DETECTOR IN HPLC AND SPECIFIC LACTATE DETERMINATION, Sensors and actuators. A, Physical, 37-8, 1993, pp. 582-586
Citations number
18
Categorie Soggetti
Engineering, Eletrical & Electronic","Instument & Instrumentation
Journal title
Sensors and actuators. A, Physical → ACNP
ISSN journal
09244247
Volume
37-8
Year of publication
1993
Pages
582 - 586
Database
ISI
SICI code
0924-4247(1993)37-8:<582:SRSAAD>2.0.ZU;2-8
Abstract
A surface plasmon resonance (SPR) sensor has been tested for its appli cation in analytical chemistry. First, the SPR sensor has been used as a universal detector in high-pressure liquid chromatography (HPLC). I t is found to be easy to stabilize and to handle, giving a broad linea r range and good reproducibility. Secondly, the SPR sensor is used to determine lactate by enzymatic reactions. It is possible to obtain som e information about the mechanism of the reactions and the SPR signals are linear with lactate concentrations under the experimental conditi ons proposed.