The potential for sexual reproduction was evaluated in the various rac
es of an international Hydrilla germplasm collection. Crosses between
dioecious and monoecious races of Hydrilla verticullata (L.f.) Royle r
esulted in seed production in 40 of the 56 crosses (71%). Seeds from 9
0% of these crosses were viable and most seedlings survived. The dioec
ious female plant, established in the US since the 1950s, has never be
en reported to produce seed, but this female was discovered to be one
of the greatest seed producers. This race has been reported to be trip
loid, but seed from four of its five crosses were viable. Triploids ar
e generally sterile, so these findings raise questions about the repor
ted ploidy level of this race. Another observation, not previously rep
orted for Hydrilla, was the occurrence of monoecious offspring from di
oecious parents. The reported lack of seed production in the US for fe
male plants of the dioecious race has been due to the absence of a via
ble pollen donor.