QUANTITATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OFBAMIPINE COMBINED WITH TRICYCLIC ANTIDEPRESSANTS AND OR ANTIPSYCHOTICS IN PHARMACEUTICAL FORMULATIONS

An advanced reserved phase High Performance Liquid Chromatographic (HP LC) method using UV detection, at 251, is presented for the simultaneo us determination of bamipine combined with tricyclic antidepressants a nd/or antipsychotics in various pharmaceutical formulations. Sample an alyses were performed on a bonded reserved-phase C-2, 5 mum, 250 X 4.6 mm ID (Lichrosorb RP-2)column using aqueous ammonium acetate at const ant ionic strength 0.015 M and acetonitrile 35 : 65 as eluent, at a fl ow rate of 0.9 ml/min. The pH was adjusted to 4.7 with acetic acid. Th e presence of acetate buffer both shortens the elution time and improv es the symmetry of the chromatographic peaks. The retention time was f or bamipine, prochlorperazine, trifluoperazine, thioridazine, chlorpro thixene, haloperidol, clomipramine, trimipramine 8.83, 11.83, 11.50, 1 1.08, 10.42, 8.05, 10.25, 9.67 min respectively. Linearity and precisi on data have been assessed. The method also involves an investigation of the dependence of the capacity ratio k' on the pH and the polarity of the mobile phase.