CORRECTION OF THE CLASS-H DEFECT IN GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR BIOSYNTHESIS IN LTK- CELLS BY A HUMAN CDNA CLONE

Authors
KAMITANI T CHANG HM ROLLINS C WANECK GL YEH ETH
Citation
T. Kamitani et al., CORRECTION OF THE CLASS-H DEFECT IN GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR BIOSYNTHESIS IN LTK- CELLS BY A HUMAN CDNA CLONE, The Journal of biological chemistry, 268(28), 1993, pp. 20733-20736
Citations number
40
Categorie Soggetti
Biology
Journal title
The Journal of biological chemistry → ACNP
ISSN journal
00219258
Volume
268
Issue
28
Year of publication
1993
Pages
20733 - 20736
Database
ISI
SICI code
0021-9258(1993)268:28<20733:COTCDI>2.0.ZU;2-6
Abstract
Previous attempts to express glycosylphosphatidylinositol-anchored pro teins in Ltk- cells have not been successful because Ltk- cells cannot synthesize N-acetylglucosamine-phosphatidylinositol, the first interm ediate in anchor biosynthesis. Using complementation cloning, we have identified a human cDNA that corrects the defect in anchor biosynthesi s and allows the expression of glycosylphosphatidylinositol-anchored p roteins in Ltk- cells. The nucleotide sequence predicts a novel cytoso lic protein of 188 amino acids.