IMMUNOCHEMICAL CHARACTERISTICS OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE SUBUNIT BINDING-PROTEIN

Authors
DEMIREVSKAKEPOVA K JUPERLIEVAMATEEVA B TODOROV I
Citation
K. Demirevskakepova et al., IMMUNOCHEMICAL CHARACTERISTICS OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE SUBUNIT BINDING-PROTEIN, Photosynthetica, 28(3), 1993, pp. 333-339
Citations number
14
Categorie Soggetti
Plant Sciences
Journal title
Photosynthetica → ACNP
ISSN journal
03003604
Volume
28
Issue
3
Year of publication
1993
Pages
333 - 339
Database
ISI
SICI code
0300-3604(1993)28:3<333:ICOR1C>2.0.ZU;2-M
Abstract
Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBPCO) and RuBPCO bi nding protein (BP) were isolated from barley leaves. RuBPCO was dissoc iated into subunits under denaturing conditions. Polyclonal antibodies against RuBPCO, RuBPCOBP and RuBPCO large subunit (LS) were raised. I mmunoblotting analyses showed that anti-RuBPCO antibodies did not cros s-react with BP. Anti-BP antibodies cross-reacted with RuBPCO small su bunit (SS) and reacted but more slightly with RuBPCO LS.